DEVELOPMENT OF SMART NANOMATERIALS FOR CANCER DIAGNOSTICS, THERANOSTICS AND IMAGE-GUIDED SURGERY
We developed a novel kind of Turn-ON probes with Near-Infrared (NIR) fluorescence mode of action. These probes were designed to fluorescently-report in real-time the presence of a certain analyte or an enzyme at a pathological site using intravital non-invasive imaging. We conjugated these probes to a drug-bearing polymer, and while they are Turned-OFF in the bloodstream following intravenous injection, they Turn-ON fluorescently at a near infrared wavelength when arriving to the tumor and releasing the drugs reporting on the (i) location of the tumor (used for diagnosis and for image-guided surgery) and (ii) drug release and activation, hence their definition as theranostic nanomedicines (therapy and diagnostics of cancer). In collaboration with the lab of Doron Shabat, a similar approach was taken for the design of chemiluminescence Turn-ON probes. This technology was licensed to Biosynth.
Theranostics Cover, Blau et al. 2017
Redy-Keisar O, Kisin-Finfer E, Ferber S, Satchi-Fainaro R*, and Shabat D*, Synthesis and Use of QCy7-derived Modular Probes for Detection and Imaging of Biologically Relevant Analytes, Nature Protocols, 9(1), 27-36 (2014). *Corresponding authors. [PDF][PubMed]
Ferber S, et al., (Satchi-Fainaro R), Polymeric nanotheranostics for real-time non-invasive optical imaging of breast cancer progression and drug release, Cancer Letters, 352(1):81-89 (2014). [PDF] [PubMed]
Blau R, et al., (Satchi-Fainaro R), Image-Guided Surgery Using Near-Infrared Turn-ON Fluorescent Nanoprobes for Precise Detection of Tumor Margins, Theranostics, 24;8(13):3437-3460 (2018). (Cover feature). [PDF] [PubMed]
Hananya N, Green O, Blau R, Satchi-Fainaro R*, Shabat D*, A Highly-Efficient Chemiluminescence Probe for Detection of Singlet Oxygen in Living Cells. Angewandte Chemie Int Ed Engl. 138(40):13438-13446 (2017) *Corresponding authors. [PDF] [PubMed]